Chelnobyl fallout:
Experimental exposure of laboratory mice and cultured HeLa cells on ground surface of 10-km zone 

Experimental scenario 

     Laboratory mice (CBA×C57BL F1 male mice) in cages made from metal net at ceiling, walls and bottom, and the cultured HeLa cells in plastic flasks were placed under a shed directly onto the ground polluted with radionuclides and exposed for 1-12 days in 10 km zone of the Chernobyl NPP.

     The dose rate of gamma-radiation was 101-108 mR/h measured by radiometer and 93-129 mR/h by individual dosimeter located under the cages and flasks. The average gamma-radiation dose rate per exposition ground was 100 mR/h (2.4 cGy/day)

    Chromosome aberrations were studied in bone marrow cells. The effects of exposure to contaminated gamma-rays on the sensitivity to the additional subsequent in vitro irradiation was also studied (data not included in the present database).
 

Reference 

     Pelevina, I. I., Afanasiev, G. G., Gotlib, V. Ya., Alferovich, A. A., Antoshchina, M. M., Ryabchenko, N. I., Saenko, A. S., Ryabtsev, I. A., and Ryabov, I. N. (1993): Exposure of cultured cells and animals (mice) within the 10-km zone of Chernobyl accident. Effect on sensitivity to subsequent irradiation. Radiat. Biol. Ecol., 33:85-92. 

Chromosome aberration analysis in bone marrow cells of mice exposed in the 10-km zone 

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Experiment Dose No. of Chromosome-type aberrations Chromatid-type aberrations
(Gy) cells Fragments Dicentrics Exchanges Fragmments Exchanges
Intact controls 0 100 0 0 0 3 0
1 day in zone 0.025 100 1 0 0 4 0
2 days in zone 0.12-0.13 100 4 0 0 2 0
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