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Materials: Human peripheral blood lymphocytes |
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Source: Cs-137 gamma-rays RBC Gammacell 40 Exactor) |
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Dose rate: 1.153 Gy/min |
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Date: August 8, 2000 and August 9, 2000 |
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Series [I] |
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Single dose: Mixture of non-irradiated and 5 Gy-irradiated blood samples (whole blood) in equal volume. |
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The mixed blood was cultured immediately (0 hr) or after incubation at 37oC for 24 hrs (24 hr). |
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Dose: [0] and [5.0] Gy |
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Incubation |
Cells |
Cu |
Cs |
Tri |
Dic |
Rc |
Ra |
aM |
F |
del |
S |
del |
Aber |
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
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0 hr |
1000 |
182 |
5 |
12 |
272 |
47 |
22 |
40 |
337 |
31 |
91 |
57 |
Dics |
842 |
70 |
50 |
28 |
8 |
2 |
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D+R |
831 |
58 |
54 |
35 |
16 |
6 |
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Del |
934 |
46 |
19 |
0 |
1 |
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All |
813 |
53 |
45 |
38 |
28 |
16 |
5 |
2 |
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24 hr |
1000 |
87 |
2 |
6 |
129 |
24 |
8 |
39 |
159 |
23 |
63 |
34 |
Dics |
925 |
34 |
23 |
12 |
5 |
1 |
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D+R |
28 |
26 |
17 |
6 |
2 |
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Del |
961 |
24 |
12 |
3 |
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All |
911 |
19 |
20 |
23 |
10 |
8 |
6 |
2 |
0 |
0 |
0 |
1 |
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Series [II] |
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Graded doses: Mixture of 10 irradiated samples in equal volume. |
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The mixed blood was cultured immediately (0 hr) or after incubation at 37oC for 24 hrs (24 hr). |
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Dose: [0.5], [1.0], [1.5], [2.0], [2.5], [3.0], [3.5], [4.0], [4.5] and [5.0] Gy |
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Incubation |
Cells |
Cu |
Cs |
Tri |
Dic |
Rc |
Ra |
aM |
F |
del |
S |
del |
Aber |
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
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0 hr |
1000 |
554 |
24 |
11 |
624 |
119 |
51 |
93 |
796 |
85 |
263 |
216 |
Dics |
579 |
262 |
106 |
41 |
11 |
1 |
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D+R |
527 |
250 |
141 |
54 |
20 |
6 |
2 |
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Del |
774 |
168 |
43 |
13 |
2 |
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All |
422 |
256 |
149 |
93 |
14 |
15 |
13 |
4 |
1 |
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24 hr |
1000 |
324 |
8 |
6 |
300 |
58 |
18 |
30 |
360 |
24 |
122 |
92 |
Dics |
764 |
171 |
55 |
9 |
1 |
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D+R |
721 |
194 |
65 |
16 |
4 |
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Del |
902 |
81 |
16 |
1 |
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All |
668 |
195 |
89 |
32 |
15 |
1 |
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Ref: |
Sasaki, M. S. (2003): Chromosomal biodosimetry by unfolding a mixed Poisson distribution: a generalized model. Int. J. Radiat. Biol., 79:83-97. |
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