Simulated non-uniform or partial-body irradiation

Mixed culture of different dose blood
 
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Materials: Human peripheral blood lymphocytes
Source: Cs-137 gamma-rays RBC Gammacell 40 Exactor)
Dose rate: 1.153 Gy/min
Date: August 8, 2000 and August 9, 2000
Series [I]
Single dose: Mixture of non-irradiated and 5 Gy-irradiated blood samples (whole blood) in equal volume.
The mixed blood was cultured immediately (0 hr) or after incubation at 37oC for 24 hrs (24 hr).
Dose: [0] and [5.0] Gy
Incubation Cells Cu Cs Tri Dic Rc Ra aM F del S del Aber 0 1 2 3 4 5 6 7 8 9 10 11
0 hr 1000 182 5 12 272 47 22 40 337 31 91 57 Dics 842 70 50 28 8 2
D+R 831 58 54 35 16 6
Del 934 46 19 0 1
All 813 53 45 38 28 16 5 2
24 hr 1000 87 2 6 129 24 8 39 159 23 63 34 Dics 925 34 23 12 5 1
D+R 28 26 17 6 2
Del 961 24 12 3
All 911 19 20 23 10 8 6 2 0 0 0 1
Series [II]
Graded doses: Mixture of 10 irradiated samples in equal volume.
The mixed blood was cultured immediately (0 hr) or after incubation at 37oC for 24 hrs (24 hr).
Dose: [0.5], [1.0], [1.5], [2.0], [2.5], [3.0], [3.5], [4.0], [4.5] and [5.0] Gy
Incubation Cells Cu Cs Tri Dic Rc Ra aM F del S del Aber 0 1 2 3 4 5 6 7 8 9 10 11
0 hr 1000 554 24 11 624 119 51 93 796 85 263 216 Dics 579 262 106 41 11 1
D+R 527 250 141 54 20 6 2
Del 774 168 43 13 2
All 422 256 149 93 14 15 13 4 1
24 hr 1000 324 8 6 300 58 18 30 360 24 122 92 Dics 764 171 55 9 1
D+R 721 194 65 16 4
Del 902 81 16 1
All 668 195 89 32 15 1
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Ref: Sasaki, M. S. (2003): Chromosomal biodosimetry by unfolding a mixed Poisson distribution: a generalized model. Int. J. Radiat. Biol., 79:83-97.
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